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Inputs: Output: Example: Inputs: <catalogue>: ICLC ; <search_field> Cell_line_name ; <query> "hela*" Output:
Accession_number ICLC HTL95023
Cell_line_name HeLa
Brief_description Species: human, Black; Tissue: cervix; Tumor: carcinoma, epitheloid
Description Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid
Depositor Obtained from ECACC
Bibliographic_reference Cancer Res 1952;12:264
Morphology epithelial-like, grown as monolayer
Culture_conditions continuous culture; MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential
amino acids; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3x104 cells/cm2;
37C, 5% CO2
Viruses Search for viruses was not performed
Properties antitumour testing; transformation; tumorigenicity; cytotoxicity; bacterial invasiveness;
virology; Susceptible to: adenovirus 3, measles, poliovirus 1, echovirus, vaccinia, arbovirus,
respiratory syncytial virus, reovirus 3, rhinovirus, Coxsackie
Release_conditions Cell line available for distribution. For non-commercial investigative use only
Hazard -
Species_validation Validated by isoenzymes: confirmed as human with AST, G6PD, MD
Tumorigenicity tumorigenic in nude mice
Karyology aneuploid
Freezing_medium Culture medium + 50% FBS + 10% DMSO
Sterility mycoplasma negative, HOECHST and PCR
Further_bibliography J Exp Med 1953;97:695; Obstet Gynecol 1971;38:945-949 [PMID: 4942173]
Accession_number ICLC HTL95015
Cell_line_name HeLa 382
Brief_description Species: human, Black; Tissue: cervix; Tumor: carcinoma, epitheloid; pBact.;
Original line: HeLa
Description Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid;
Transfected with pBact, expression vector under control of the human beta actin promotor,
resistant to G418S. Control to HeLa 422 and HeLa 432.; Original line: HeLa
Depositor Dr.U. Pfeffer, Molecular biology Unit, IST at ABC, Genoa Italy.
Bibliographic_reference -
Morphology epithelial-like, grown as monolayer
........
Inputs: Output: Example: Inputs: Output:
Culture_conditions continuous culture; MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential
amino acids; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3x104
cells/cm2; 37C, 5% CO2
Culture_conditions continuous culture; DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures
1:2-1:6 using trypsin/EDTA; seed at 1-3x104 cells/cm2; 37C, 5% CO2.
Culture_conditions continuous culture; DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures
1:2-1:6 using trypsin/EDTA; seed at 1-3x104 cells/cm2, 37C, 5% CO2.
Culture_conditions continuous culture; DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures
1:2-1:6 using trypsin/EDTA; seed at 1-3x104 cells/cm2; 37C, 5% CO2.
Culture_conditions continuous culture; Ham's F12 + 10% FBS + 2mM L-Glutamine; Semiadherent cell line,
adaptable to growth in suspension (spinner) culture. Maintain culture between 3 and 9x105 cells/ml;
split 1:2-1:4. 37C, 5% CO2
Inputs: Output: Example: Inputs: <catalogue>: ICLC ; <search_field> Cell_line_name ; <query> "hela*" Output: ICLC:ICLC HTL95023 ICLC:ICLC HTL95015 ICLC:ICLC HTL95014 ICLC:ICLC HTL95016 ICLC:ICLC HTL95020 |
CABRI
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