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IST Bioinformatics Web Services: CABRI: Human and animal cell lines
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Inputs: Output: Example: Inputs: <libs>: ICLC_SP_DSMZ_MUTZ ; <name>: hela* Output: DSMZ_MUTZ:ACC 57 DSMZ_MUTZ:ACC 161 ICLC:ICLC HTL95023 ICLC:ICLC HTL95015 ICLC:ICLC HTL95014 ICLC:ICLC HTL95016 ICLC:ICLC HTL95020 Inputs: Output: Example: Input: <libs>: ICLC_SP_DSMZ_MUTZ ; <text>: rhabdomyosarcoma Output: DSMZ_MUTZ:ACC 250 DSMZ_MUTZ:ACC 493 DSMZ_MUTZ:ACC 491 DSMZ_MUTZ:ACC 489 DSMZ_MUTZ:ACC 592 DSMZ_MUTZ:ACC 263 ICLC:ICLC HTL99002 ICLC:ICLC HTL97021 Inputs: Output: Example: Inputs: <libs>: ICLC ; <ID>: ICLC_SP_HTL03003 Output:
Accession_number ICLC HTL03003
Cell_line_name 293
Brief_description Species: human; Tissue: kidney, embryonic; Transformed by: adenovirus 5
Description Species: human; Tissue: kidney, embryonic; Transformed by: adenovirus 5
Depositor Obtained from ECACC
Bibliographic_reference J Gen Virol 1977;36:59-74 [PMID: 886304]
Morphology epithelial-like, grown as monolayer
Culture_conditions continuous culture; DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids;
Split cultures 1:3-1:6 using trypsin/EDTA; 37?C, 5% CO2. The viability may be low after thawing and
the cells may take up to 7 days to attach. The cells should be frozen at not less than 8 x 10^6 /ampoule
and thawed with 20% FBS
Viruses Search for viruses was not performed
Release_conditions Cell line available for distribution. For non-commercial investigative use only
Hazard Handle as Biosafety Level 2 containment
Freezing_medium 90% FBS + 10% DMSO
Sterility mycoplasma negative, HOECHST and PCR
Validation_assays Multiplex PCR: tested against human, rat, mouse primers.
Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further_bibliography Virology 1977;77:319-329 [PMID: 841862]
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CABRI
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