<br>CHO-K1<br>

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

CHO-K1 (hamster, Chinese, ovary)

DSMZ catalog code: ACC 110
SPECIES: hamster, Chinese
TISSUE/ORGAN: ovary
Susceptibility to viruses: arbovirus * Getah arbovirus * mengovirus * vesic. stomatitis (Indiana)

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
mycoplasma negative, DAPI, broth-agar, hybridization
Validated by: cytogenetics 1 (near diploid with 12% polyploidy; 22(20-23); exhibits distinctive CHO karyology) * DNA fingerprinting (same Hinf I-(gtg)5 DNA profile as CHO-DHFR) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

CHO-K1 (hamster, Chinese, ovary)

ECACC catalog code: 85051005
SPECIES: hamster, Chinese
TISSUE/ORGAN: ovary
Susceptibility to viruses: arbovirus * Getah arbovirus * mengovirus * vesic. stomatitis (Indiana)

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
mycoplasma negative, HOECHST and culture
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 61, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

CHO-K1 (hamster, Chinese, ovary)

ICLC catalog code: ATL98003
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: hamster, Chinese (female, adult)
TISSUE/ORGAN: ovary
Derived from: CHO
DEPOSITOR: Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: The cells have an absolute requirement for L-proline

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
Ham's F12 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:4-1:10 using trypsin/EDTA; seed at 1-2 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as hamster with MD, NP)

Availability in cell line catalogues: ECACC: 85051005, DSMZ: ACC 110

Bibliographic references:

J Exp Med 1958;108:945

CHO-K1 (hamster, Chinese, ovary)

Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: hamster, Chinese Karyology: hypodiploid, modal number 20
TISSUE/ORGAN: ovary
Derived from: CHO
Susceptibility to viruses: arbovirus * Getah arbovirus * mengovirus * vesic. stomatitis (Indiana)
PROPERTIES: genetics; nutrition; transfection; toxicity screening; cell biology; virology; cytotoxicity; bacterial cytotoxicity; gene expression

Available in the following LABORATORY:

Istituto di Scienze Farmacologiche, Universita' degli Studi (MIUFC, Milano)
Ham's F12 + DMEM (1:1) + 10% FBS + Antibiotics; Freezing medium: Culture medium + 20% FBS + 10% Glycerol; 37C, 5% CO2, split 1-4 , mycoplasma negative
Validated by: biochemical assays * cellular assays

Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

CHO-K1 (hamster, Chinese, ovary)

Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: hamster, Chinese Karyology: hypodiploid, modal number 20
TISSUE/ORGAN: ovary
Original line: CHO
Susceptibility to viruses: arbovirus * Getah arbovirus * mengovirus * vesic. stomatitis (Indiana)
PROPERTIES: genetics; nutrition; transfection; toxicity screening; cell biology; virology; cytotoxicity; bacterial cytotoxicity; gene expression

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)

Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

CHO-K1 (hamster, Chinese, ovary)

Available in the following LABORATORY:

Universita' La Sapienza (RMUPG, Roma)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 5% CO2
Proline-requiring

Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

CHO-K1 (hamster, Chinese, ovary)

Available in the following LABORATORY:

Dip. Biologia Molecolare, Universita' degli Studi (SIUVI, Siena)
MEM modified + 10% FBS + Antibiotics + NaHCO3; Freezing medium: MEM modified + 20% FBS + 7.5% DMSO; 37C, 5% CO2, split 1-4 , mycoplasma negative, enzyme immunoassay (EIA)
Validated by: chromosome analysis

Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

CHO-K1 (hamster, Chinese, ovary)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CCL 61, ECACC: 85051005, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990

For information, get in touch with:

Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy Tel: +39-0105737-289, Fax: +39-0105737-295
Email: assunta.manniello@istge.it