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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
DSMZ catalog code: ACC 169
SPECIES: human, Caucasian
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
mycoplasma negative - mycoplasma negative, hybridization assay
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, ICLC: HTL97023, IZSBS: BS TCL 87
Bibliographic references:
- Biochimie 1986;68:1035-1040 - PMID: 3096381
- Biol Cell 1983;47:323-330
- Exp Cell Res 1992;200:97-104 - PMID: 1563496
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
ECACC catalog code: 86010202
SPECIES: human, Caucasian
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 37, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87
Bibliographic references:
- Biochimie 1986;68:1035-1040 - PMID: 3096381
- Biol Cell 1983;47:323-330
- Exp Cell Res 1992;200:97-104 - PMID: 1563496
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
ICLC catalog code: HTL97023
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 72 years old) Karyology: modal number 96
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
tumorigenic in nude mice
DEPOSITOR: Dr A. Corsini, Pharmacological Sciences Institute, Milan, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: lipid production; small bowel biology; metabolism; lipoprotein
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2. After thawing, cells might grow slowly. 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR - mycoplasma contamination eliminated with BM-cyclin; negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with MD, NP)
Availability in cell line catalogues: ECACC: 86010202, DSMZ: ACC 169, IZSBS: BS TCL 87
Bibliographic references:
- Biochimie 1986;68:1035-1040 - PMID: 3096381
- Biol Cell 1983;47:323-330
- Exp Cell Res 1992;200:97-104 - PMID: 1563496
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
IZSBS catalog code: BS TCL 87
Continuous culture, grown as monolayer
Epithelial-like morphology
SPECIES: human, Caucasian (male, 72 years old) Karyology: 2n = 46 in 50 cells
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
tumorigenic
DEPOSITOR: obtained from ATCC
Distribution is subjected to authorization by the assignee
Patent strain
PROPERTIES: toxicology studies; expressing xenobiotic metabolizing enzymes
Available in the following LABORATORY:
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Istituto Zooprofilattico Sperimentale (IZSBS, Brescia)
MEM (EBSS) + 15% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 18 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, MPI, PEPB)
Availability in cell line catalogues: ATCC: HTB-37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023
Bibliographic references:
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 72 years old) Karyology: modal number 96
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: lipid prod.; small bowel biology; metabolism; lipoprotein; tumorigenicity; cell biology; biochemistry; biophysics
Available in the following LABORATORY:
-
Pharmacia Upjhon (MIECC, Nerviano (MI))
MEM (EBSS) + 20% FBS + 1% non-essential amino acids; Freezing medium: MEM + 20% FBS + 10% Glycerol; 37C, 5% CO2, split 2-3
Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87
Bibliographic references:
- Biochimie 1986;68:1035-1040 - PMID: 3096381
- Biol Cell 1983;47:323-330
- Exp Cell Res 1992;200:97-104 - PMID: 1563496
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 72 years old) Karyology: modal number 96
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: lipid prod.; small bowel biology; metabolism; lipoprotein; tumorigenicity; cell biology; biochemistry; biophysics
Available in the following LABORATORY:
-
Istituto Superiore di Sanità (RMSTC, Roma)
DMEM + 5% FBS + Antibiotics + 2mM L-Glutamine; Freezing medium: DMEM + 20% FBS + 10% DMSO; 37C, 5% CO2, split 1-9 , mycoplasma negative
Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87
Bibliographic references:
- Biochimie 1986;68:1035-1040 - PMID: 3096381
- Biol Cell 1983;47:323-330
- Exp Cell Res 1992;200:97-104 - PMID: 1563496
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 72 years old) Karyology: modal number 96
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: lipid prod.; small bowel biology; metabolism; lipoprotein; tumorigenicity; cell biology; biochemistry; biophysics
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87
Bibliographic references:
- Biochimie 1986;68:1035-1040 - PMID: 3096381
- Biol Cell 1983;47:323-330
- Exp Cell Res 1992;200:97-104 - PMID: 1563496
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-295
Email: assunta.manniello@istge.it