<br>Sp2/0-Ag14<br>

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

Sp2/0-Ag14 (mouse, BALB/c, hybrid, myeloma)

Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: mouse, BALB/c (female)
TISSUE/ORGAN: hybrid
TUMOR: myeloma
Original line: P3X63Ag8 x BALB/c mouse spleen cell
Transformed by: fusion with anti SRBC spleen cells by PEG
tumorigenic in syngeneic animals
PROPERTIES: Ig non synthesized; resistant to 8-azaguanine; fusion partner; monoclonal antibody prod.

Available in the following LABORATORY:

Istituto Nazionale Riposo e Cura Anziani (INRCA) (ANIIR, Ancona)
DMEM + 10% FBS + Antibiotics + 2mM L-Glutamine + D-Glucose/L + 1mM Na pyruvate; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative

Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, ECACC: 85072401, DSMZ: ACC 146

Bibliographic references:

J Immunol 1981;126:317-321 - PMID: 6935293
Nature 1978;276:269-270 - PMID: 714156

Sp2/0-Ag14 (mouse/mouse, myeloma)

DSMZ catalog code: ACC 146
SPECIES: mouse/mouse
TUMOR: myeloma
Transformed by:

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
mycoplasma negative, DAPI, broth-agar, hybridization
Validated by: isoenzymes (confirmed as mouse with IEF of LDH, NP, PEP B)

Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, ECACC: 85072401

Bibliographic references:

J Immunol 1981;126:317-321 - PMID: 6935293
Nature 1978;276:269-270 - PMID: 714156

Sp2/0-Ag14 (mouse, BALB/c, hybrid, myeloma)

ECACC catalog code: 85072401
SPECIES: mouse, BALB/c
TISSUE/ORGAN: hybrid
TUMOR: myeloma
Transformed by:

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
mycoplasma negative, HOECHST and culture
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, DSMZ: ACC 146

Bibliographic references:

J Immunol 1981;126:317-321 - PMID: 6935293
Nature 1978;276:269-270 - PMID: 714156

Sp2/0-Ag14 (mouse, BALB/c, hybrid, myeloma)

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)

Availability in cell line catalogues: ATCC: CRL 1581, ATCC: CRL 1581, ECACC: 85072401, DSMZ: ACC 146

Bibliographic references:

J Immunol 1981;126:317-321 - PMID: 6935293
Nature 1978;276:269-270 - PMID: 714156

For information, get in touch with:

Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy Tel: +39-0105558-289, Fax: +39-0105558-295
Email: assunta.manniello@istge.it