<br>P3X63Ag8U.1<br>

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

P3X63Ag8U.1 (mouse, BALB/c, plasmacytoma)

ECACC catalog code: 94120802
SPECIES: mouse, BALB/c
TUMOR: plasmacytoma

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
mycoplasma negative, HOECHST and culture
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1597, ICLC: ATL96012

Bibliographic references:

Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551
J Exp Med 1979;150:580 - PMID: 90108

P3X63Ag8U.1 (mouse, BALB/c, plasmacytoma)

Monoclonal continuous culture, grown as suspension
lymphoid morphology
SPECIES: mouse, BALB/c
TUMOR: plasmacytoma
PROPERTIES: antibody prod.; fusion partner; protein; biochemistry

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (GEIIB, Genova)
DMEM + 10% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-5
Established in Albert Einstein Institute,SHARFF NY

Availability in cell line catalogues: ATCC: CRL 1597, ECACC: 94120802, ICLC: ATL96012

Bibliographic references:

Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551
J Exp Med 1979;150:580 - PMID: 90108

P3X63Ag8U.1 (mouse, BALB/c, myeloma)

ICLC catalog code: ATL96012
Continuous culture, grown as suspension
lymphoblast morphology
SPECIES: mouse, BALB/c (female)
TUMOR: myeloma
Derived from: P3X63Ag8
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: fusion partner; HGPRT- and HAT sensitive; does not synthesize gamma1 chains, produces kappa light chains which are not secreted

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split 1:5-1:10; maintain cultures between 3 and 9x105 cells/ml; rapid cell death occurs at >1x106 cells/ml. 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with AST, G6PD)

Availability in cell line catalogues: ECACC: 94120802

Bibliographic references:

Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551

For information, get in touch with:

Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy Tel: +39-0105737-289, Fax: +39-0105737-295
Email: assunta.manniello@istge.it