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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HL97002
Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human
TISSUE/ORGAN: skin, keratinocyte
DEPOSITOR: Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy.
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: The cells show a good expression of cytochrome P450-dependent enzymatic activities, and could be a good cellular model for cytotoxicity studies; toxicology.
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-5x104 cells/cm2; 37C, 5% CO2. Doubling time: 21 hours. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with MD, NP)
Availability in cell line catalogues: IZSBS: BS CL 143
Bibliographic references:
- Arch Dermatol Res 1976;256(3):255-260 - PMID: 990102
- Arch Dermatol Res 1976;261(1):27-31
IZSBS catalog code: BS CL 143
Continuous culture, grown as monolayer
Epithelial-like morphology
SPECIES: human
TISSUE/ORGAN: skin, keratinocyte
DEPOSITOR: obtained from Dipartimento di Medicina Sperimentale, Università degli Studi, Genova (I)
PROPERTIES: used in biochemical and toxicological studies
Available in the following LABORATORY:
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Istituto Zooprofilattico Sperimentale (IZSBS, Brescia)
DMEM + 7% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, MPI, PEPB, AST, LD)
Availability in cell line catalogues: ICLC: HL97002
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-295
Email: assunta.manniello@istge.it