<br>MOLT-4<br>

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

ECACC catalog code: 85011413
SPECIES: human
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
Susceptibility to viruses: alpha viruses * measles

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
mycoplasma negative, HOECHST and culture
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

Monoclonal continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (GEIIB, Genova)
RPMI 1640 + 10% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 10% CO2, split 1-5

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, peripheral blood, leukemia, T cell)

ICLC catalog code: HTL99025
Continuous culture, grown as suspension
round cells, single or in clumps, lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, T cell
Derived from: a patient with acute lymphoblastic leukemia (ALL) in relapse
DEPOSITOR: Dr G. Canti, Pharmacology Dept, Milan, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: G->A mutation at codon 248 of the p53; p53 is not expressed; CD1+ CD2+ CD3+ CD4+ CD5+ CD6+ CD7+ CD19- EBV-; high level of terminal deoxynucleotidyl transferase

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 20% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 5-10 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)

Availability in cell line catalogues: ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231
J Natl Cancer Inst 1980;65;715:718 - PMID: 6932523
Proc Natl Acad Sci USA 1990;87:7555-7559 - PMID: 16992228

MOLT-4 (human, peripheral blood, leukemia)

IZSBS catalog code: BS TCL 85
Continuous culture, grown as suspension
Lymphoblast-like morphology
SPECIES: human
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia
DEPOSITOR: obtained from ATCC
Distribution is subjected to authorization by the assignee.
Patent strain

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale (IZSBS, Brescia)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, MD, MPI)

Availability in cell line catalogues: ATCC: CRL-1582, ICLC: HTL99025, ECACC: 85011413, DSMZ: ACC 362

Bibliographic references:

J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)

HeLa markers. At the moment the availability of this cell line is not sure

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

Available in the following LABORATORY:

Centro Trapianto Midollo Osseo e Divisione Ematologia (PSHTM, Pesaro)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + Antibiotics + 1mM Na pyruvate; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-2

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

Available in the following LABORATORY:

Istituto Superiore di Sanità (RMSVI, Roma)
RPMI 1640 + 10% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-7

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

Available in the following LABORATORY:

Dip.Medicina Sperimentale e Scienze Biochimiche, Universita' degli Studi (RMUFM, Roma)
RPMI 1640 + 15% FBS + Antibiotics + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative
Validated by: T cell markers

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

For information, get in touch with:

Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy Tel: +39-0105737-289, Fax: +39-0105737-295
Email: assunta.manniello@istge.it