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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ECACC catalog code: 85112702
SPECIES: rat, buffalo
TISSUE/ORGAN: liver
TUMOR: hepatoma
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 144, DSMZ: ACC 106, ICLC: ATL98014
Bibliographic references:
- Cell Biochem Funct 1988;6:245-250 - PMID: 3191583
- Chem Biol Interact 1988;65:629-634
- Exp Cell Res 1991;196:330-336 - PMID: 1716586
- In Vitro 1970;6:32 - PMID: 4399803
- J Cell Biol 1969;40:236-247 - PMID: 4177659
- J Cell Res 1969;40:236
- Science 1970;170:533 - PMID: 4908238
Monoclonal continuous culture, grown as monolayer
epithelial morphology
SPECIES: rat, buffalo (male) Karyology: chromosome fragments and breaks
TISSUE/ORGAN: liver
TUMOR: hepatoma
Derived from: Morris hepatoma 7795
tumorigenic in syngeneic animals
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
Cell line available under some conditions.
PROPERTIES: serum albumin prod.; serum complement components prod.; monooxygenases expression; tyrosine aminotransferase synthesis; enzymology; metabolism; toxicity screening; cytotoxicity; xenobiotic metabolism; hormone studies
Available in the following LABORATORY:
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Istituto Patologia Generale, Universita' degli Studi (GEUCC, Genova)
Ham's F10 + 7.5% NBCS + 10 HS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-5 Passage: 70 , mycoplasma negative, HOECHST
Chromosome markers: 1 large chromosome with submedian centromere; 1-2 large chromosomes with median centromeres. Derived from hepatoma induced by N-2-fluorenylpthalamic acid.
Validated by: monooxygenase expression and induction
Availability in cell line catalogues: ATCC: CCL 144, ECACC: 85112702, DSMZ: ACC 106, ICLC: ATL98014
Bibliographic references:
- Cell Biochem Funct 1988;6:245-250 - PMID: 3191583
- Chem Biol Interact 1988;65:629-634
- Exp Cell Res 1991;196:330-336 - PMID: 1716586
- In Vitro 1970;6:32 - PMID: 4399803
- J Cell Biol 1969;40:236-247 - PMID: 4177659
- J Cell Res 1969;40:236
- Science 1970;170:533 - PMID: 4908238
ICLC catalog code: ATL98014
Continuous culture, grown as monolayer
epithelial; the cells grow in large clusters morphology
SPECIES: rat
TISSUE/ORGAN: liver
TUMOR: hepatoma
Derived from: transplantable Morris hepatoma (n.7795, Buffalo strain)
DEPOSITOR: Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells synthesize and secrete serum albumin, C9, tyrosine aminotransferase; monooxigenases expression; toxicity screening; xenobiotic metabolism; drug metabolism assays; hepatocyte studies
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-2 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
cell harvest is about 10 x 10^6 cells/25 cm2 flask
Validated by: isoenzymes (confirmed as rat with AST, MD)
Availability in cell line catalogues: ECACC: 85112702, DSMZ: ACC 106
Bibliographic references:
- Cell Biochem Funct 1988;6:245-250 - PMID: 3191583
- Exp Cell Res 1991;196:330-336 - PMID: 1716586
- J Cell Biol 1969;40:236-247 - PMID: 4177659
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-295
Email: assunta.manniello@istge.it