<br>HTC<br>

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

HTC (rat, buffalo, liver, hepatoma)

ECACC catalog code: 93120108
SPECIES: rat, buffalo
TISSUE/ORGAN: liver
TUMOR: hepatoma

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
mycoplasma negative, HOECHST and culture
Validated by: DNA fingerprinting * isoenzymes ((species confirmation))

Availability in cell line catalogues: ICLC: ATL95006

Bibliographic references:

Adv Cancer Res 1965;9:227 - PMID: 4285261
ATLA 1988;16:32
Proc Natl Acad Sci U S A 1966;56:269 - PMID: 4381784

HTC (rat, buffalo, ascitic fluid, hepatoma)

ICLC catalog code: ATL95006
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: rat, buffalo (male) Karyology: extra chromosomes 1 and 13
TISSUE/ORGAN: ascitic fluid
TUMOR: hepatoma
Derived from: Morris hepatoma 7288C
tumorigenic in syngeneic animals
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: biochemical markers; enzymology; toxicity screening; tumorigenicity; cytotoxicity; xenobiotic metabolism; dexamethasone phosphate and hydrocortisone receptor

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium 70% + 20% Horse serum + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as rat with MD, NP)

Availability in cell line catalogues: ECACC: 93120108

Bibliographic references:

Adv Cancer Res 1965;9:227 - PMID: 4285261
ATLA 1988;16:32
Proc Natl Acad Sci USA 1966;56:269 - PMID: 4381784

HTC (rat, buffalo, ascitic fluid, hepatoma)

Continuous culture, grown as suspension
epithelial-like morphology
SPECIES: rat, buffalo (male) Karyology: extra chromosomes 1 and 13
TISSUE/ORGAN: ascitic fluid
TUMOR: hepatoma
Original line: Morris hepatoma 7288C
tumorigenic in syngeneic animals
PROPERTIES: biochemical markers; enzymology; toxicity screening; tumorigenicity; cytotoxicity; xenobiotic metabolism; dexamethasone p receptor

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Contains inducible tyrosine aminotransferase.

Availability in cell line catalogues: ECACC: 93120108, ICLC: ATL95006

Bibliographic references:

Adv Cancer Res 1965;9:227 - PMID: 4285261
ATLA 1988;16:32
Proc Natl Acad Sci U S A 1966;56:269 - PMID: 4381784

For information, get in touch with:

Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy Tel: +39-0105737-289, Fax: +39-0105737-295
Email: assunta.manniello@istge.it