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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Caucasian (female, 36 years old) Karyology: pseudodiploid
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, promyelocytic
tumorigenic in nude mice
Susceptibility to viruses: HIV-1 * HTLV-1
PROPERTIES: phagocytosis; antitumour testing; differentiation; pharmacodynamics; cloning
Available in the following LABORATORY:
-
Clinica Pediatrica, Ospedale S.Orsola-Malpighi (BOHIC, Bologna)
RPMI 1640 + 20% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-6 , mycoplasma negative, DAPI and HOECHST
Split every 3-4 days
Validated by: B cell markers
Availability in cell line catalogues: ATCC: CCL 240, DSMZ: ACC 3, ICLC: HTL95010
Bibliographic references:
- Blood 1979;54:713-733 - PMID: 288488
- Blood 1988;71:242-247 - PMID: 3422031
- Nature 1977;270:347-349 - PMID: 271272
- Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884
DSMZ catalog code: ACC 3
SPECIES: human
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute myeloid
Susceptibility to viruses: HIV-1 * HTLV-1
Available in the following LABORATORY:
-
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
mycoplasma negative, DAPI and broth-agar
Validated by: cytogenetics 1 (hypotetraploid karyotype without clear mode, 1.5% polyploidy; 82(78-88)<4n>XX, -X, -X, -2, -3, -4, -5, -8, -9, -10, -14, -16, -17, -17, +3m, der(6)t(6;?)(q25;?)/dup(6)(q23;qter)x2, del(9)(p22), del(11)(q22/23), der(16)t(16;17)) * cytogenetics 2 ((q22/23;q21-22)x2; high frequency of subclonal ch 1 rearrangements) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD14- CD15+ CD19- CD33+ HLA-DR-) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (not detected (RDDP assay))
Availability in cell line catalogues: ATCC: CCL 240, ICLC: HTL95010
Bibliographic references:
- Blood 1979;54:713-733 - PMID: 288488
- Blood 1988;71:242-247 - PMID: 3422031
- Nature 1977;270:347-349 - PMID: 271272
- Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884
ICLC catalog code: HTL95010
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Caucasian (female, 36 years old) Karyology: pseudodiploid
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, promyelocytic
tumorigenic in nude mice
Susceptibility to viruses: HIV-1 * HTLV-1
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: phagocytosis; antitumour testing; differentiation; pharmacodynamics; cloning
Available in the following LABORATORY:
-
Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10; maintain cultures between 5-9x105 cells/ml; 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
After treatment with DMSO the cells differentiate along the granulocytic line (after 4 days in 1,25% DMSO, 72% of the cells become CD11b positive).
Validated by: isoenzymes (confirmed as human with MD, MPI, NP)
Availability in cell line catalogues: DSMZ: ACC 3
Bibliographic references:
- Blood 1979;54:713-733 - PMID: 288488
- Nature 1977;270:347-349 - PMID: 271272
- Proc Natl Acad Sci USA 1978;75:2458-2462 - PMID: 276884
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Caucasian (female, 36 years old) Karyology: pseudodiploid
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, promyelocytic
tumorigenic in nude mice
Susceptibility to viruses: HIV-1 * HTLV-1
PROPERTIES: phagocytosis; antitumour testing; differentiation; pharmacodynamics; cloning
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 240, DSMZ: ACC 3, ICLC: HTL95010
Bibliographic references:
- Blood 1979;54:713-733 - PMID: 288488
- Blood 1988;71:242-247 - PMID: 3422031
- Nature 1977;270:347-349 - PMID: 271272
- Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Caucasian (female, 36 years old) Karyology: pseudodiploid
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, promyelocytic
tumorigenic in nude mice
Susceptibility to viruses: HIV-1 * HTLV-1
PROPERTIES: phagocytosis; antitumour testing; differentiation; pharmacodynamics; cloning
Available in the following LABORATORY:
-
Centro di Riferimento Oncologico (PNIOS, Aviano)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 5% CO2 Passage: 510
Obtained from Dr. Mertelsmann Sloan Kettering New York
Availability in cell line catalogues: ATCC: CCL 240, DSMZ: ACC 3, ICLC: HTL95010
Bibliographic references:
- Blood 1979;54:713-733 - PMID: 288488
- Blood 1988;71:242-247 - PMID: 3422031
- Nature 1977;270:347-349 - PMID: 271272
- Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Caucasian (female, 36 years old) Karyology: pseudodiploid
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, promyelocytic
tumorigenic in nude mice
Susceptibility to viruses: HIV-1 * HTLV-1
PROPERTIES: phagocytosis; antitumour testing; differentiation; pharmacodynamics; cloning
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 240, DSMZ: ACC 3, ICLC: HTL95010
Bibliographic references:
- Blood 1979;54:713-733 - PMID: 288488
- Blood 1988;71:242-247 - PMID: 3422031
- Nature 1977;270:347-349 - PMID: 271272
- Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Caucasian (female, 36 years old) Karyology: pseudodiploid
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, promyelocytic
tumorigenic in nude mice
Susceptibility to viruses: HIV-1 * HTLV-1
PROPERTIES: phagocytosis; antitumour testing; differentiation; pharmacodynamics; cloning
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 240, DSMZ: ACC 3, ICLC: HTL95010
Bibliographic references:
- Blood 1979;54:713-733 - PMID: 288488
- Blood 1988;71:242-247 - PMID: 3422031
- Nature 1977;270:347-349 - PMID: 271272
- Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-295
Email: assunta.manniello@istge.it