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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ECACC catalog code: 85011430
SPECIES: human, Caucasian
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ICLC: HTL95005
Bibliographic references:
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
Cell line available under some conditions.
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology
Available in the following LABORATORY:
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Istituto Patologia Generale, Universita' degli Studi (GEUCC, Genova)
MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-5 , mycoplasma negative, HOECHST
Aneuploidies, partial trisomies, translocations, pericentric inversions, chrom. 1 rearrangements
Validated by: monooxygenase expression and induction
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005
Bibliographic references:
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
ICLC catalog code: HTL95005
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, 15 years old)
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
DEPOSITOR: Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells are reported to produce: alpha-fetoprotein, albumin, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, alpha1-antichimotrypsin, haptoglobin, ceruloplasmin, plasminogen, C3,C4, C3 act., fibrinogen, alpha1-acid glycop., alpha2-HS glycoprotein, beta lipoprotein and retinol binding protein; xenobiotic metabolism, drug metabolism assays, toxicity screening, hepatocyte studies
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-3 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with MD, MPI)
Availability in cell line catalogues: IZSBS: BS TCL79, ECACC: 85011430
Bibliographic references:
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
IZSBS catalog code: BS TCL79
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology
Available in the following LABORATORY:
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Istituto Zooprofilattico Sperimentale (IZSBS, Brescia)
MEM (EBSS) + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 94 , mycoplasma negative, culture
Kariotype: 2n=55 in 50 cells. Patent strain, distribution is subjected to authorization by the assignee. Tumor: hepatoblastoma.
Availability in cell line catalogues: ATCC: HB 8065, ECACC: 85011430, ICLC: HTL95005
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology
Available in the following LABORATORY:
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Pharmacia Upjhon (MIECC, Nerviano (MI))
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: DMEM + 20% FBS + 10% Glycerol; 37C, 5% CO2, split 2-3 , mycoplasma negative
Epithelial-like morphology
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005
Bibliographic references:
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology
Available in the following LABORATORY:
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Universita' degli Studi (PGUPG, Perugia)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% Glycerol; 37C, 5% CO2
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005
Bibliographic references:
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005
Bibliographic references:
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005
Bibliographic references:
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-295
Email: assunta.manniello@istge.it