<br>HeLa<br>

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

HeLa (human, Black, cervix, carcinoma, epitheloid)

DSMZ catalog code: ACC 57
SPECIES: human, Black
TISSUE/ORGAN: cervix
TUMOR: carcinoma, epitheloid
Susceptibility to viruses: adenovirus 3 * arbovirus * Coxsackie * echovirus * measles * poliovirus 1 * reovirus 3 * respiratory syncytial virus * rhinovirus * vaccinia

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
mycoplasma negative, DAPI, broth-agar, hybridization
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of GPDH, MDH, NP) * reverse transcriptase (reverse trancriptase not detected)

Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, ECACC: 93021013, ICLC: HTL95023

Bibliographic references:

Cancer Res 1952;12:264
J Exp Med 1953;97:695
J Gen Virol 1983;64:825 - PMID: 6834007
J Virol 1978;28:444 - PMID: 214572
Obstet Gynecol 1971;38:945-949 - PMID: 4942173

HeLa (human, Black, cervix, carcinoma, epitheloid)

ECACC catalog code: 93021013
SPECIES: human, Black
TISSUE/ORGAN: cervix
TUMOR: carcinoma, epitheloid
Susceptibility to viruses: adenovirus 3 * arbovirus * Coxsackie * echovirus * measles * poliovirus 1 * reovirus 3 * respiratory syncytial virus * rhinovirus * vaccinia

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
mycoplasma negative, HOECHST and culture
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, DSMZ: ACC 57, ICLC: HTL95023

Bibliographic references:

Cancer Res 1952;12:264
J Exp Med 1953;97:695
J Gen Virol 1983;64:825 - PMID: 6834007
J Virol 1978;28:444 - PMID: 214572
Obstet Gynecol 1971;38:945-949 - PMID: 4942173

HeLa (human, Black, cervix, carcinoma, epitheloid)

ICLC catalog code: HTL95023
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Black (female, 31 years old) Karyology: aneuploid
TISSUE/ORGAN: cervix
TUMOR: carcinoma, epitheloid
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 3 * arbovirus * Coxsackie * echovirus * measles * poliovirus 1 * reovirus 3 * respiratory syncytial virus * rhinovirus * vaccinia
DEPOSITOR: Obtained from ECACC
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: antitumour testing; transformation; tumorigenicity; cytotoxicity; bacterial invasiveness; virology

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3x104 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, G6PD, MD)

Availability in cell line catalogues: IZSBS: BS TCL20, ECACC: 93021013, DSMZ: ACC 57

Bibliographic references:

Cancer Res 1952;12:264
J Exp Med 1953;97:695
Obstet Gynecol 1971;38:945-949 - PMID: 4942173

HeLa (human, Black, cervix, carcinoma, epitheloid)

IZSBS catalog code: BS TCL20
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Black (female, 31 years old) Karyology: aneuploid
TISSUE/ORGAN: cervix
TUMOR: carcinoma, epitheloid
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 3 * arbovirus * Coxsackie * echovirus * measles * poliovirus 1 * reovirus 3 * respiratory syncytial virus * rhinovirus * vaccinia
PROPERTIES: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale (IZSBS, Brescia)
MEM (EBSS) + 10% FBS; Freezing medium: Culture medium + 10% Glycerol; 37C, 5% CO2 , mycoplasma negative, culture

Availability in cell line catalogues: ATCC: CCL 2, ECACC: 93021013, DSMZ: ACC 57, ICLC: HTL95023

HeLa (human, Black, cervix, carcinoma, epitheloid)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Black (female, 31 years old) Karyology: aneuploid
TISSUE/ORGAN: cervix
TUMOR: carcinoma, epitheloid
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 3 * arbovirus * Coxsackie * echovirus * measles * poliovirus 1 * reovirus 3 * respiratory syncytial virus * rhinovirus * vaccinia
PROPERTIES: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology

Available in the following LABORATORY:

Boehringer Mannheim Italia S.p.A. (MIEOS, Monza (MI))
MEM (EBSS) + 10% Human serum + 1% non-essential amino acids; Freezing medium: Culture medium + 15% Human serum + 5% Glycerol; 37C, 5% CO2 , mycoplasma negative
Validated by: isoenzymes

Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, ECACC: 93021013, DSMZ: ACC 57, ICLC: HTL95023

Bibliographic references:

Cancer Res 1952;12:264
J Exp Med 1953;97:695
J Gen Virol 1983;64:825 - PMID: 6834007
J Virol 1978;28:444 - PMID: 214572
Obstet Gynecol 1971;38:945-949 - PMID: 4942173

HeLa (human, Black, cervix, carcinoma, epitheloid)

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)
mycoplasma negative

Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, ECACC: 93021013, DSMZ: ACC 57, ICLC: HTL95023

Bibliographic references:

Cancer Res 1952;12:264
J Exp Med 1953;97:695
J Gen Virol 1983;64:825 - PMID: 6834007
J Virol 1978;28:444 - PMID: 214572
Obstet Gynecol 1971;38:945-949 - PMID: 4942173

HeLa (human, Black, cervix, carcinoma, epitheloid)

Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Black (female, 31 years old) Karyology: aneuploid
TISSUE/ORGAN: cervix
TUMOR: carcinoma, epitheloid
tumorigenic in nude mice
Susceptibility to viruses: adenovirus 3 * arbovirus * Coxsackie * echovirus * measles * poliovirus 1 * reovirus 3 * respiratory syncytial virus * rhinovirus * vaccinia
PROPERTIES: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology

Available in the following LABORATORY:

ENEA, CRE-Casaccia (RMEBA, Roma)
DMEM + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split1-9 , mycoplasma negative

Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, ECACC: 93021013, DSMZ: ACC 57, ICLC: HTL95023

Bibliographic references:

Cancer Res 1952;12:264
J Exp Med 1953;97:695
J Gen Virol 1983;64:825 - PMID: 6834007
J Virol 1978;28:444 - PMID: 214572
Obstet Gynecol 1971;38:945-949 - PMID: 4942173

For information, get in touch with:

Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy Tel: +39-0105737-289, Fax: +39-0105737-295
Email: assunta.manniello@istge.it