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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ECACC catalog code: 88030401
SPECIES: human, Caucasian
TISSUE/ORGAN: skin
TUMOR: malignant melanoma
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CRL 1424, ICLC: HTL99001
Bibliographic references:
ICLC catalog code: HTL99001
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (male, 31 years old)
TISSUE/ORGAN: skin
TUMOR: melanoma
tumorigenic in nude mice
DEPOSITOR: Dr S. Barlati, Biology and Genetics Dept, University of Brescia, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cells produce tissue-type plasminogen activator (tPA)
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4x10^4 cells/cm2;37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: ECACC: 88030401
Bibliographic references:
- FEBS Lett 1995;363:170-174 - PMID: 7537224
- Invasion Metastasis 1995;15:22-23 - PMID: 7672929
- Pediatr Res 1978;12:485
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-295
Email: assunta.manniello@istge.it