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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
DSMZ catalog code: ACC 35
SPECIES: human
TISSUE/ORGAN: bladder
TUMOR: carcinoma
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
mycoplasma negative, DAPI, broth-agar, hybridization
Validated by: cytogenetics 1 (hypotriploid with 17% polyploidy; 62(47-64)<3n>XX, -Y, -4, -5, -10, -13,-13, -15, -18, -19, -21, -21, +4mar, der(1)t(1;?)(p21;?), der(3)t(3;?)(p22;?), del(6)(q23), der(9)t(9;?)(p12;?), der (11)t(5;11)(q14;p12), ?der(12)t(12;) * cytogenetics 2 (i(13q), i(14q), der(17)t(17;?)(p11/12;?), del(22)(q13); carries large submetacentric marker with q arm resembling tandem dup(7); 9p12 is a recurrent breakpoint in bladder carcinoma.) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HTB 9, ECACC: 90110524, ICLC: HTL01017
Bibliographic references:
- Proc Natl Acad Sci U S A 1985;82:1526-1530 - PMID: 3871951
ICLC catalog code: HTL01017
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, 68 year old)
TISSUE/ORGAN: bladder
TUMOR: carcinoma
tumorigenic in nude mice
DEPOSITOR: Dr M. Musso, Oncology, Biology and Genetic Dept, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: GM-CSF, IL-1, IL-6 production
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:4-1:8 using trypsin/EDTA (8-10 min.); 37C, 5% CO2 , mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: ECACC: 90110524, DSMZ: ACC 35
Bibliographic references:
- J Natl Cancer Inst 1977;59:221-226 - PMID: 77210034
- Natl Cancer Inst Monogr 1978;49:5-9 - PMID: 79156922
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-295
Email: assunta.manniello@istge.it