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TUMOR: sarcoma, osteogenic
ECACC catalog code: 89090403
Susceptibility to viruses: canine hepatitis * herpes * parainfluenza
Description: Canine Poodle primary osteosarcoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding 3-5x10,000 cells/cm2 using 0.25% trypsin/0.02% EDTA; 5% CO2; 37C.
Karyotype: 2n = 78
Derived from an osteosarcoma metastatic to the lung in an 11 year old female poodle. Used extensively in virus studies. Susceptible to canine herpes, canine parainfluenza and infectious canine hepatitis.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 183
- J Gen Virol 1974;25:21 - PMID: 4372315
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293