COS-1

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

COS-1 (monkey, African green, kidney)

SPECIES: monkey, African green
TISSUE/ORGAN: kidney

DSMZ catalog code: ACC 63
Morphology: fibroblast-like, adherent monolayers
TRANSFORMED BY: SV40
Description: African green monkey kidney established from CV-1 Simian cells (cercopithecus aethiops) which were transformed by an origin-defective mutant of SV-40; cells are host cells for the propagation of pure populations of recombinant SV virus; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS) confirmed as of non-human primate origin with IEF of AST, NP, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HCV-, HBV-, HIV-, HTLV-I/II-
Depositor: Dr. R. Riebe, Friedrich-Loeffler-Institute, Insel Riems, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS split confluent cultures 1:3 to 1:10 every 3-7 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm 2 in 10 ml medium at 37C with 5% CO2 about 5 x 106 cells/80 cm 2; doubling time of ca. 48 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotetraploid simian karyotype with 4% polyploidy; 102(95-108)<4n>; structural rearrangements present) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile (identical to COS-7)) * isoenzymes (confirmed as of primate origin with IEF of NP, PEP B)

Availability in cell line catalogues: ATCC: CRL 1650, IZSBS: BS CL95, ECACC: 88031701, ICLC: ATL01002

Bibliographic references:

Cell 1981;23:175-182 - PMID: 6260373