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TISSUE/ORGAN: ascitic fluid
TUMOR: adenocarcinoma, ovary
ECACC catalog code: 93072111
Description: Human adenocarcinoma
Viruses: See Properties
Depositor: Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 5-10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Cells can be maintained in the absence of serum for several passages.
Derived from the ascitic fluid from 73-year old Chinese female with ovarian cancer. The relatively small epithelial cells grow loosely attached to surfaces and suspended in media as single cells, variously sized strings and clumps of adherent cells. Derived from the same patient as COLO 720L.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293