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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: human
TUMOR: melanoma
DSMZ catalog code: ACC 264
Morphology: adherent fibroblastic, but not limited to monolayer growth
Description: human melanoma established in 1985 from the soft tissue metastasis of a malignant melanoma from a 47-year-old woman confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. G. E. Moore, Colorado Oncology Foundation, Denver, Colorado, USA
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90-95% RPMI 1640 + 5-10% FBS split confluent culture 1:5 to 1:10 using trypsin/EDTA; change medium twice weekly; seed out at ca. 2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 25 x 106 cells/175 cm 2;
doubling time of about 34 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (flat-moded, hypotetraploid karyotype with 8% polyploidy; 83-92<4n>XXXX, -1, +3, -4, +7, +7, -14, -15, -15, -16, -18, - 20, -20, +3m; del(3)(p11)x1-2, del(3)(q13.3), add(7)(q35)x2, del(9)(p22)x2, add(10)(p11)x1-2, add(10)(p13),) * cytogenetics 2 (add(14)(p11), add(19)(p13.3)x2, add(21)(p11), add(22)(q13)x4; extensive subclonal variation of chs 1, 5 and 7; 9p- recurrent in melanoma) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin-, desmin-, endothel-, GFAP-, neurofilament+ (10%), vimentin+ (100%)) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase not detected)
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it