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SPECIES: mouse, RIII
TISSUE/ORGAN: mammary gland
TUMOR: mammary tumor
ECACC catalog code: 87091701
Susceptibility to viruses: bovine papilloma
Description: Mouse RIII mammary tumour
Viruses: See Properties
Depositor: Dr T Ohno, Riken Gene Bank, Tsukuba, JAPAN
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transfection and virus studies: Bovine papilloma virus
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Subclone of parent line C127. The cells support the growth of Bovine Papilloma Virus and has been used for DNA transfection studies.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1616
- J Virol 1978;26:291-298 - PMID: 26810
- Virology 1980;103:369-375 - PMID: 6247821
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293