Clone M-3

Version 4.0.200811

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Clone M-3 (mouse, (CxDBA) F1, skin, melanoma)

SPECIES: mouse, (CxDBA) F1
TISSUE/ORGAN: skin
TUMOR: melanoma

ECACC catalog code: 87081806
Morphology: Epithelial
Susceptibility to viruses: herpes simplex * pseudorabies * vaccinia * vesic. stomatitis (Indiana)
Description: Mouse (CxDBA)F1 melanoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Melanin Applications: Cloning studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F10 + 2mM Glutamine + 10% Horse Serum (HS) + 5% Fetal Bovine Serum (FBS) or 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:4 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 43; Karyotype: Hypertetraploid
Established from a Cloudman S91 melanoma in a male mouse; the cells produce melanin.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 53.1, IZSBS: BS TCL37

Bibliographic references:

Science 1966;154:1186 - PMID: 4288399