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SPECIES: human, Caucasian
PATHOLOGY: citrullinemia*215700 (OMIM record)
ECACC catalog code: 90102532
Description: Human Caucasian skin
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Genetic studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C. Finite lifespan up to 30 passages.
Karyotype: 2n = 46
Human diploid cell line derived from a young Caucasian female with Citrullinemia. The cells grow poorly when citrulline is substituted for arginine in the recommended medium and they incorporate very little 14C citrulline into cell protein. The cell line also has an abnormally low affinity for citrulline as a substrate in an in vivo assay of arginosuccinate synthetase.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 76
- Proc Natl Acad Sci U S A 1967;57:829
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293