3T3

Version 4.0.200811

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3T3 (mouse, Swiss albino, embryo)

IZSBS catalog code: BS CL 82
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: mouse, Swiss albino Karyology: 2n = 40 in 48 cells
TISSUE/ORGAN: embryo
non tumorigenic
Susceptibility to viruses: herpes simplex * polyoma virus * Sendai * SV40 * vesic. stomatitis (Indiana)
PROPERTIES: Conctat inhibited. The cell line is readily transformed by two oncogenic viruses, polyoma and SV40.

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale (IZSBS, Brescia)
MEM (EBSS) + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 116, mycoplasma negative, culture
Validated by: isoenzymes (confirmed as mouse with NP, G6PD, PEPB, AST, LD)

Availability in cell line catalogues: ATCC: CCL 92, DSMZ: ACC 173, ICLC: ATL95002

Bibliographic references:

Arch Virol 1984;80:47 - PMID: 6200094