3T3 L1

Version 4.0.200811

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3T3 L1 (mouse, Swiss albino, embryo)

SPECIES: mouse, Swiss albino
TISSUE/ORGAN: embryo

ECACC catalog code: 86052701
Morphology: Fibroblast-like. Adipose like when confluent
Description: Mouse embryo
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Calf Serum (CS). Split confluent cultures 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm2 in a 75 cm2 flask using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Never allow the culture to become fully confluent and subculture every 3 days.
Passages: 16; Karyotype: 2n = 40; Anueploid with unstable karyotype
3T3 L1 is a continuous strain of 3T3 developed through clonal isolation. Cells undergo a pre-adipose to adipose-like conversion as they progress from rapidly dividing to a confluent and contact inhibited state. A high serum content in the culture medium reportedly enhances the rapidity and degree of fat accumulation.

Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 92.1, IZSBS: BS CL 100

Bibliographic references:

Cell 1974;1:113 - PMID: 4473270
Cell 1974;3:127 - PMID: 4426090