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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL 08004
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human
TISSUE/ORGAN: brain
TUMOR: glioblastoma
Transfected by: Transfected with wt EGFR Back To Transfection WebPage
DEPOSITOR: Dr T. Florio, DOBIG, Pharmacology Dept, University of Genoa, Italy,
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium 45% + 50% FCS + 5% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Handle as Biosafety Level 2 containment
Originator of cell line: dr. W. K. Cavenee
Validated by: Multiplex PCR (tested again human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Bibliographic references:
- Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7727-31 - PMID: 8052651
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it