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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: ATL07002
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: mouse, CD-1 transgenic (female)
TISSUE/ORGAN: mammary gland
TUMOR: adenocarcinoma
Derived from: lung metastasis
TRANSFORMED BY: MMTVneu oncogene Back To Oncogene Agents' WebPage
DEPOSITOR: Dr M.G. Sacco, Human Genome Dept, ITB-CNR, Milan, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
William's medium + 15% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 10% FBS + 10% DMSO; split confluent cultures 1:2-1:4; 37C, 5% CO2 Passage: 19, mycoplasma negative, HOECHST and PCR
fluorescent cells. Cells also grow well with the heat inactivated serum
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers)
Bibliographic references:
- Cell Prolif. 2006 Dec;39(6):611-22. - PMID: 17109643
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it