Chang Liver

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

Chang Liver (human, liver)

SPECIES: human
TISSUE/ORGAN: liver

ECACC catalog code: 88021102
Morphology: Epithelial
Susceptibility to viruses: adenovirus 3 * influenza * poliovirus 1 * poliovirus 2 * poliovirus 3 * vesic. stomatitis (Indiana)
Description: Human liver, HeLa characteristics
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus, biochemical and replication studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 260; Karyotype: Modal No 70
One of the first cell lines to be derived from non-malignant tissue. Used in virology, biochemistry and transplantation related to malignancy. Cells tend to pile up in cultures with high population density. This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 13, IZSBS: BS CL14

Bibliographic references:

J Exp Med 1961;113:95
Natl Cancer Inst Monogr 1962;7:249
Proc Soc Exp Biol Med 1954;87:440