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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL06006
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: mouse, NIH Swiss
TISSUE/ORGAN: embryo
Original line: TK- NIH/3T3 cells
Transfected by: (pPAM3) and HSV thymidine kinase Back To Transfection WebPage
DEPOSITOR: Dr M. Dominici, Oncology Dept, University of Modena e Reggio Emilia, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: Packaging line for retroviruses
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM (4.5g/l glucose) + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 5% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA, 37C, 5% CO2 Passage: 3, mycoplasma negative, DAPI and HOECHST
Handle under Biosafety Level 2 containment
amphotropic packaging cell line
Availability in cell line catalogues: ECACC: 89032007
Bibliographic references:
- J Biol Chem 1996;271: 13968-13975 - PMID: 8662928
- J Virol 1997;71: 345-353 - PMID: 8985356
- Mol Cell Biol 1986; 6: 2895-2902 - PMID: 3785217
- N Engl J Med 1990;323: 570-578 - PMID: 2381442
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it