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ICLC catalog code: IST05001
Primary culture, grown as monolayer
Derived from: umbilical cord vein
DEPOSITOR: Dr A. Albini, Exp. Oncology A Dept, IST, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: % of CD31 positive cells: >90%
Available in the following LABORATORY:
Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
Medium 199 + 10% FBS + 2mM L-Glutamine + heparin 100 mg/L + aFGF 10ug/L + bFGF 10ug/L + EGF 10ug/L + hydrocortisone 1mg/L; Freezing medium: 90% FBS + 10% DMSO; split the cells 1:2-1:3 using cold trypsin/EDTA (no more than one minute), seed at 6x10^3 cells/cm2, 37° C, 5% CO2, mycoplasma negative, DAPI
The cells reach at least passage 12; better results are guaranteed at low passages. Each ampoule contains about 5x10^5 cells. After thawing the viability of the cells may be low. The medium should be kept at 4°C, it should never be heated neither left too long at room temperature. The flasks must be presoaked with gelatin 1% in PBS for 30 minutes. After gelatin coating, rinse once with cold PBS. Do not reuse flask. Donors are tested for HBV, HCV, HIV. HUVEC are available from single donors (male or female), and the sex is specified.
- Microvasc Res 2004;68(2):110-8 - PMID: 15313120
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293