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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL05004
Continuous culture, grown as suspension
lymphoblast, single and in small clusters growth morphology
SPECIES: human (female)
TISSUE/ORGAN: lymphocyte, B
PATHOLOGY: paroxysmal nocturnal hemoglobinuria+311770 (OMIM record)
TRANSFORMED BY: Epstein-Barr
DEPOSITOR: Dr R. Notaro, Human Genetic Dept IST, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: GPI-
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 4 mML-Glutamine + 20% FBS ; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 350-400 x 10^3 cells/ml; duplication time 48-60 h; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Handle under Biosafety Level 2 containment
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it