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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL03001
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, 58 year old)
TISSUE/ORGAN: lung
TUMOR: carcinoma
DEPOSITOR: Dr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cells could synthesize lecithin and are positive for keratin
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:4 using trypsin/EDTA; 37°C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Availability in cell line catalogues: ECACC: 86012804, IZSBS: TCL 101
Bibliographic references:
- Int J Cancer 1976;17:62-70 - PMID: 175022
- J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it