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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: mouse, 129
TISSUE/ORGAN: embryo
ECACC catalog code: 95011018
Morphology: Small, tightly packed
Description: Mouse embryonic stem cell
Viruses: See Properties
Depositor: Dr A Smith, AFRC Centre for Genome Research, Edinburgh, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Gene targeting, Gene trapping, in vitro differentiation
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
GMEM + 2mM Glutamine + 100 units/ml DIA/LIF + 0.05mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 i.e. seeding at 4x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 16;
Karyotype: 40XY
The germ-line competent cell line CGR8 was established from the inner cell mass of a 3.5 day male pre-implantation mouse embryo (Mus musculus, strain 129). These pluripotent cells retain the ability to participate in normal embryonic development. Differentiation of CGR8 cells is inhibited by the pleiotropic cytokine Differentiation Inhibiting Activity (DIA) which is identical to Leukaemia Inhibiting Factor (LIF). Addition of DIA/LIF allows culture of CGR8 without the use of feeder layers.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Bibliographic references:
- Development 1990;110:1341 - PMID: 2129226
- J Tiss Cult Meth 1991;13:89
- Proc Natl Acad Sci U S A 1994;91(10):4303-4307
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it