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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL01016
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (female, 58 year old)
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma, colorectal
tumorigenic in nude mice
DEPOSITOR: Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: CEA, IL-6, IL-10 and mucin production
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + 15% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split cultures 1:4-1:6 using trypsin/EDTA (for at least 10 min.), or by scraping; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
The cells are difficult to detach
Validated by: HLA-DP beta (confirmed as human by PCR)
Bibliographic references:
- In Vitro 1976;12:180-191 - PMID: 76166575
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it