CFPAC-1

Version 4.0.200811

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CFPAC-1 (human, Caucasian, pancreas, adenocarcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: pancreas
TUMOR: adenocarcinoma

ECACC catalog code: 91112501
Morphology: Epithelial
Description: Human Caucasian pancreatic adenocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Cystic fibrosis trans-membrane regulator (CFTR) Applications: Cystic fibrosis studies, genetic analysis

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
IDMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 22; Karyotype: Hyperdiploid, (65-75 chromosomes)
CFPAC-1 is a ductal pancreatic adenocarcinoma derived by differential trypsinisation of explant cultures from a metastatic lesion in the liver of a 26 year old caucasian male with cystic fibrosis (CF). The cell line expresses cystic fibrosis trans-membrane regulator (CFTR) and ion transport properties consistent with the CF defect. CFPAC-1 cells show an epithelial morphology and polarisation with apical microvilli. Expression of cytokeratin and oncofoetal antigens characteristic of pancreatic duct cells have been described. The cells express the CF gene and show the most common CF mutation, deletion of three nucleotides result in a phenylalanine-508 deletion. CFPAC-1 cells are also tumorigenic in nude mice.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1918, ICLC: HTL01019

Bibliographic references:

Am J Med 1988;85:592 - PMID: 3177424
Proc Natl Acad Sci U S A 1990;87:4012-4016 - PMID: 1692630