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TUMOR: leukemia, myelomonocytic
ECACC catalog code: 92110206
Description: Human lymphoblast
Viruses: See Properties
Depositor: Not registered
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Fc, complement Products: Surface Immunoglobulin ( Ig), MHC Class II Applications: T cell replacement factor (TRF) assays, B cell differentiation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37C.
Derived from a patient with myelomonocytic leukaemia and subsequently exposed to Epstein Barr Virus (EBV). This line is EBV positive and therefore presumably derived from a normal B lymphocyte. This cell line is also positive for surface Ig, Fc receptor, complement receptor, surface Ia and EBNA. Responds to T cell replacement factor by increasing the number of IgG secreting cells. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: TIB 190
- Br J Haematol 1982;51:595-604 - PMID: 6980663
- J Immunol 1981;127:412 - PMID: 6972960
- J Immunol 1981;128:675
- J Immunol 1982;129:1367 - PMID: 6980930
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293