U937(CD59+)

Version 4.0.200811

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U937(CD59+) (human, Caucasian, lymphoma, histiocytic)

SPECIES: human, Caucasian
TUMOR: lymphoma, histiocytic

ECACC catalog code: 95102435
Morphology: Lymphoid
Description: Human Caucasian histiocytic lymphoma
Viruses: See Properties
Depositor: Dr C W Van den Berg, Department of Medical Biochemistry, University of Wales, Cardiff, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of complement inhibition by CD59

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 4mM Glutamine + 2mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37C.
The cell line CD59(+) was derived from the lymphoma cell line U937 (ECACC No. 85011440) by a single limited dilution step. Although the cells express CD59, mAb directed against CD59 did not enhance the killing of this cell line. Together with results from Western blot analysis these results indicate that CD59(+) cells express a dysfunctional and structurally abnormal form of CD59.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmatio)

Bibliographic references:

Immunology 1994;81:637 - PMID: 7518799

For information, get in touch with:

Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it