CCRF-SB

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

CCRF-SB (human, Caucasian, peripheral blood, leukemia, acute lymphoblastic)

SPECIES: human, Caucasian
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute lymphoblastic

ECACC catalog code: 89090405
Morphology: Lymphoblastoid
Description: Human Caucasian acute lymphoblastic leukaemia
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: HLA profile studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Maintain cultures between 3x10,000 to 1x100,000 cells/ml; 5% CO2; 37C. Cultures can tolerate high densities i.e. 1-2x1,000,000 cells/ml.
Karyotype: 2n = 46
A B lymphoblastoid line obtained from the peripheral blood of an 11 year old boy with acute lymphoblastic leukaemia. Appear free of virus particles by electron microscopy. Do not synthesise immunoglobulins. Grown in continuous spinner culture for 6 mths. HLA profile: A1, A2, B12, B17, Cw2.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 120, IZSBS: BS TCL12

Bibliographic references:

Cancer Res 1967;27:2479 - PMID: 4170381
Monograph 1967;21