CAPAN-2

Version 4.0.200811

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CAPAN-2 (human, Caucasian, pancreas, adenocarcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: pancreas
TUMOR: adenocarcinoma

DSMZ catalog code: ACC 245
Morphology: adherent, epithelial-like cells growing in uniform monolayers
Description: human pancreas adenocarcinoma established from the tumor of a 56-year-old Caucasian man with pancreas adenocarcinoma in 1975 confirmed as human with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from DKFZ Tumorbank, Heidelberg, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85% RPMI 1640 + 15% FBS split semi-confluent (2/3 to 3/4 confluency) culture 1:10 every 7 days using trypsin/EDTA (for 10-20 min); seed out at ca. 1-2 x 106 cells/80 cm 2 , adherence may require several hours or overnight at 37C with 5% CO2 cell harvest of about 20 x 106 cells/175 cm 2; doubling time of about 50-70 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypertriploid karyotype with 6% polyploidy; 68- 73<3n>XX, -Y, +1, +1, +4, +5, +7, +11, -12, -14, -17, -18, -22, +3mar; del(1)(p11p33)x2, add(11)(p11), der(11)t(?8;11)(q12;p11)x2, i(14q)x2, add(21)(p11)x2, 1-2DM(24%); sdl with +1,) * cytogenetics 2 (i(1q)x2, del(1)(q11)) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+, desmin-, endothel-, GFAP-, neurofilament-, vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: HTB 80

Bibliographic references:

Cancer Res 1986;46:5810-5815 - PMID: 3019537