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ECACC catalog code: 90110508
TRANSFORMED BY: SV40
Description: Human placenta (post crisis); SV40 transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Human chorionic gonadotropin (hCG)
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin; EDTA; 5% CO2; 37C.
Derived from 3A (tPA-30-1); unlike the original the cells they have unlimited lifespan in culture. The parent line has been transformed using the temperature sensitive SV40 ts1730 virus. NB: Normal placental functions expressed at 40C (non-permissive temperature). Sub-culture should be drawn every 2-3 days at this temperature.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1584
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293