Calu-6

Version 4.0.200811

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Calu-6 (human, Caucasian, lung, carcinoma, anaplastic)

ICLC catalog code: HTL97003
Continuous culture, grown as monolayer
epithelial-like and round cells morphology
SPECIES: human, Caucasian (female, 61 years old)
TISSUE/ORGAN: lung
TUMOR: carcinoma, anaplastic
Derived from: Derived from anaplastic carcinoma, probably of lung origin
tumorigenic in nude mice
DEPOSITOR: Dr A. Ardizzoni, Medical Oncology Dept, IST, Genoa, Italy.
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split subconfluent cultures 1:5-1:10 using trypsin/EDTA (1-2 min); seed at 3-5 x 104 cells/cm2. 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, G6PD)

Bibliographic references:

Cancer Res 1988;48:3716-3719 - PMID: 3378214
Human tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080