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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL95029
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 69 years old)
TISSUE/ORGAN: kidney
TUMOR: carcinoma
tumorigenic in nude mice
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
McCoy's 5A + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2 Passage: 52, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, MD, NP, PepB)
Availability in cell line catalogues: IZSBS: BS TCL8, DSMZ: ACC 54
Bibliographic references:
- Human tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159
- J Natl Cancer Inst 1977;49:5
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
- Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it