CACO-2

Version 4.0.200811

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CACO-2 (human, Caucasian, colon, adenocarcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma

DSMZ catalog code: ACC 169
Morphology: epithelial adherent cells; after splitting cells start to grow in colonies
Description: human colon adenocarcinoma established from the primary colon tumor (adenocarcinoma) of a 72-year-old Caucasian man in 1974 confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Prof. A. Bacher, Technical University of Munich, Munich, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% MEM (with Earle's salts) + 20% FBS + 1x non-essential amino acids cells grow slowly; split confluent culture 1:6 to 1:10 every 5-7 days using trypsin/EDTA or, if necessary, a cell scraper; after thawing cells might start slowly to take up growth; seed out at ca. 1 x 106 cells/80 cm 2 in 10 ml medium at 37C with 5% CO2 about 3-6 x 106 cells/80 cm 2 or ca. 1.0 x 105 cells/cm 2; doubling time of ca. 80 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule; contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiologcal culture, RNA hybridization, PCR assay;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, ICLC: HTL97023, IZSBS: BS TCL 87

Bibliographic references:

Biochimie 1986;68:1035-1040 - PMID: 3096381
Biol Cell 1983;47:323-330
Exp Cell Res 1992;200:97-104 - PMID: 1563496
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080