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SPECIES: human, Caucasian
ECACC catalog code: 86010202
Description: Human Caucasian colon adenocarcinoma
Viruses: See Properties
Depositor: Dr J Clarke, AVRI, Pirbright, UK
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). The addition of Bovine Insulin has been reported to be useful where there has been slow growth of cells. Split sub-confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. NB: During routine subculture the cells should always be subcultured before they achieve confluence.
Karyotype: Hypertetraploid, modal No 96
Isolated from a primary colonic tumour in a 72-year-old Caucasian male using the explant culture technique. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: HTB 37, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87
- Biochimie 1986;68:1035-1040 - PMID: 3096381
- Biol Cell 1983;47:323-330
- Exp Cell Res 1992;200:97-104 - PMID: 1563496
- J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293