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TUMOR: glial tumor
ECACC catalog code: 85040101
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
Description: Rat glial tumour
Viruses: See Properties
Depositor: Dr T J Hill, Dept. of Microbiology, University of Bristol, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: S-100 protein Applications: Biochemical and virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 42
Derived from a rat glial tumour induced by N-nitrosomethylurea. The cells produce S-100 protein which is found in the brain and also in brain tumours from both human and in other animals. The cell line is capable of performing this organ specific function over a period of a year and is capable of producing elevated levels of glyceryl phosphate dehydrogenase activity when stimulated by glucocorticoides. The synthesis of S-100 is dependent on the level of confluence and increases ten fold as cells grow to confluence.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 107, IZSBS: BS TCL5, ICLC: ATL95007
- Fed Proc 1968;27:720
- Science 1968;161:370 - PMID: 4873531
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293