C-4 II

Version 4.0.200811

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C-4 II (human, Caucasian, cervix, carcinoma, cervical)

SPECIES: human, Caucasian
TISSUE/ORGAN: cervix
TUMOR: carcinoma, cervical

ECACC catalog code: 88102701
Morphology: Epithelial colonies with hemicysts
Description: Human Caucasian cervical carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Waymouth's MB 752/1 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 107; From cervical carcinoma of 41 year Caucasian female. Cells form spreading predominantly monolayered, epithelial colonies with hemicysts which are characteristic of transporting epithelia and respond to crowding by separation and shedding. These cells have retained properties of basal cell differentiation in long term culture and exhibit G6PD type B. C-4 II cells have been reported to contain human papilloma virus 18 (HPV-18) DNA sequences and to express HPV-18 RNA.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1595

Bibliographic references:

J Cell Biol 1980;85:325-336 - PMID: 6768754
J Natl Cancer Inst 1962;28:605-627
J Natl Cancer Inst 1969;43:151-173 - PMID: 5796380
J Natl Cancer Inst 1969;43:175-190

For information, get in touch with:

Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it