C32

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

C32 (human, skin, melanoma, amelanotic)

SPECIES: human
TISSUE/ORGAN: skin
TUMOR: melanoma, amelanotic

ECACC catalog code: 87090201
Morphology: Epithelial
Description: Human amelanotic melanoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No 45
C32 belongs to the C32r type cultures with the marker M2r substituting the original ring marker M2R. Tumours can be produced in nude mice after 1-2 months (1,000,000 cells injected).
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1585, IZSBS: BS TCL 150

Bibliographic references:

Cancer Res 1973;33:2042 - PMID: 4725368
J Natl Cancer Inst 1978;61:277 - PMID: 277716