C2C12

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

C2C12 (mouse, C3H, muscle)

SPECIES: mouse, C3H
TISSUE/ORGAN: muscle

ECACC catalog code: 91031101
Morphology: Myoblast
Description: Mouse C3H muscle myoblast
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10-15% Fetal Bovine Serum (FBS). Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C .Do not allow cells to reach confluence.
Passages: 13; Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.
Research council deposit: No; Price Code: A;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1772, IZSBS: BS CL 148

Bibliographic references:

Nature 1977;270:725-727 - PMID: 563524
Science 1985;230:758-766 - PMID: 2414846