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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: human, Caucasian
TISSUE/ORGAN: skin
PATHOLOGY: cri du chat syndrome#123450 (OMIM record)
ECACC catalog code: 90112604
Morphology: Fibroblast
Description: Human Caucasian skin, Cri du Chat syndrome
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Genetic studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
McCoy's 5a + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Passages: 18;
Karyotype: 2n = 46
Derived from biopsy of an 11 year old Caucasian male with Cri du Chat syndrome. Cells have a limited lifespan of approximately 35 passages. Autoradiographic studies demonstrated that a distinction could be made between chromosome 4 and 5 in the Cri du chat syndrome by alternative DNA replication pattern and length of long and short arms.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 123
Bibliographic references:
- Am J Hum Genet 1967;19:399 - PMID: 6026933
- Cytogenetics 1966;5:137 - PMID: 5921496
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it