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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: rat
TISSUE/ORGAN: liver
TUMOR: hepatoma
ECACC catalog code: 94101906
Morphology: Epithelial
Description: Rat hepatoma [HGPRT-]
Viruses: See Properties
Depositor: Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Gene expression analysis.
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Coon's modified Ham's F12 + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split semi-confluent cultures 1:50 i.e. seeding at 2x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Saturation density is reached at 10x10,000,000,000 cells/10cm² dish.
Passages: 9;
Karyotype: Hyperdiploid, 52 Chromosomes
Full name Faof1C2 is a dedifferentiated variant of Fao. These cells continue to express tyrosine aminotransferase, but the liver-specific markers are mostly absent.
Research council deposit: No;
Price Code: C;
Bibliographic references:
- Biochimie 1974;56:1603-1611 - PMID: 4157008
- Genes dev 1991;5(6):1042-1056 - PMID: 2044952
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it