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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL01001
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (female, 11 years old)
TISSUE/ORGAN: bone
TUMOR: osteosarcoma
DEPOSITOR: Dr G. Di Domenico, Biology and Cellular and Molecular Pathology Dept, Naples, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: epidermal growth factor (EGF) and transforming growth factor beta (type 1 and type 2) receptor expression; positive for alkaline phosphatase
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA, 37C, 5% CO2 Passage: 15, mycoplasma negative, HOECHST and culture
Validated by: HLA-DP beta (confirmed as human by PCR)
Molecular authentication: Amelogenin: X ; D18S51: 15 ; D21S11: 28,30 ; D8S1179: 10,12 ; FGA: 22,25 ; TH01: 6,9 ; VWA: 18
Availability in cell line catalogues: ATCC: CRL 8303, IZSBS: BS TLC 90, DSMZ: ACC 243, ECACC: 89050205
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it